In living plants, there are various water-soluble organic/inorganic chemicals. Such water-soluble chemicals can be moved in the sample preparation process of trimming, fixation,sectioning, and drying. To visualize their distribution in the living state, cryo time-of-flight secondary ion mass spectrometry (cryo-TOF-SIMS) was applied to freeze-fixed plant samples. Cryo-TOF-SIMS images were obtained from the transverse and radial surface of freeze-fixed Ginkgo biloba. One of the target chemicals, coniferin is a promising candidate for the lignin precursor; however, the detailed distribution in planta was unclear. Coniferin distribution visualized at the transverse and radial surfaces of freeze-fixed stem of G. biloba suggested that coniferin was stored in the vacuoles, and showed good agreement with the assimilation timing of coniferin to lignin in differentiating xylem. Olivil 4,4’-di-O-β-D-glucopyranoside is a kind of lignan glucoside and abundant in the stem of G. biloba. The distribution of olivil-diglucoside was also visualized by cryo-TOF-SIMS. Olivil-diglucoside distributed mainly in the living tissues. The quaternary ammonium alkaloid, salicifoline in freeze-fixed stem of Magnolia kobus was also visualized by cryo-TOF-SIMS. Most of salicifoline was stored in the bark region. In the bark region, salicifoline was detected only from the living tissues. In the xylem region, salicifoline distribution varied with the cell wall formation and lignification stages.

Cryo-TOF-SIMS visualization of water-soluble chemicals in plant

253rd National Meeting (2017)

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