Glycan profiling is a promising area for biomarker discovery, but many protein candidates are present at ng/ml levels or lower. This makes direct glycoprotein profiling via LC-MS impossible. Immunoprecipitation methods can be used to increase analyte concentration, but all suffer from nonspecific binding. In an effort to perform glycan profiling on prostate specific antigen serum samples, ferrimagnetic nanoparticles were coated with silica using a modified Stöber process. After further surface modification, activator generated electron transfer atom transfer radical polymerization was used to grow a chromatographic quality acrylamide copolymer surface. The high density copolymer reduced biofouling and increased active antibody binding 5x over Dynabeads. Nanoparticles were characterized using transmission electron microscopy and a superconducting quantum interference device.

Silica coated ferrimagnetic nanoparticles in trace glycoprotein recovery for LC-MS

253rd National Meeting (2017)

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