Introduction Both, vitexin and isovitexin are C-glycosyl flavonoids found in edible plants, herbs, fruits and vegetables. To identify the α-glucosidase inhibitors from an edible plant, Ficus deltoidea and evaluate the in vitro and in vivo inhibitor of these flavonoids. The α-glucosidase enzyme from Bacillus stearotherophilus (3 U/mL) was prepared in phosphate buffer (pH 6.5). The mixture of α-glucosidase enzyme solution, tests samples and buffer were pipetted and mixed in a 96 well microtiter plate. The mixture was incubated for 15 min at 37 0C and later 4-nitrophenol α-D-glucopyranoside (NPG) was added. The increment of absorbance due to the hydrolysis of NPG byα-glucosidase was measured at the wavelength of 405 nm. Sucrose loaded rodents were used to evaluate the in vivo α-glucosidase inhibition. The in vitro α-glucosidase IC50 values of vitexin and isovitexin were 4.1 and 6.7 ug/mL. Sucrose loaded normoglycemia mice and streptozotocin (STZ) induced diabetic rat administered with either vitexin or isovitexin exhibited significant blood glucose reduction. Both the C-glycosyl flavonoids have potential as an α-glucosidase inhibitors.

In vitro and in vivo a-glucosidase inhibition of vitexin and isovitexin

253rd National Meeting (2017)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.