Over the past decade, considerable progress has been made in improving the effectiveness and efficiency of generating highly-productive recombinant CHO cell lines. These improvements are now the underpinning of bioprocess development and involve both advancements in cell line generation and cell culture process development. While these efforts have been primarily centered on driving cell culture productivity, more recently, focus has turned to approaches to impact product quality. Specifically, host engineering capabilities have been directed to successfully edit CHO cell lines to improve product quality profiles of bio-therapeutic proteins. Given the labor-intensive, inefficient and time-consuming nature of such capability application, significant need exists to find creative approaches to maximize the effectiveness, while minimizing the impact of such efforts to timelines. This presentation will describe potential approaches to maximizing the effectiveness of host cell engineering and reducing the time to successfully impact bio-therapeutic product quality profiles.

Effective and efficient approaches to cell line engineering in CHO cells

253rd National Meeting (2017)

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