First generation biosensor development is significant because the strategy can be readily adapted to many different, clinically-relevant analytes with specific enzymatic reactions to help provide selectivity. Oxidation of hydrogen peroxide, a by-product of said enzymatic reactions, at a platinum electrode allows for amperometric sensing of the targeted analyte in real-time. Layer-by-layer (LbL) xerogel-based biosensor designs have been developed at macro-electrodes where each layer of a specific material is optimized for sensitivity and selectivity. LbL systems have been explored for glucose sensing, as a model system, as well as uric acid sensing for early detection of pregnancy-induced hypertension and preeclampsia risk. In order for such sensors to be feasible in clinical use, the LbL strategy must be successfully miniaturized to wire electrodes that can potentially function <i>in vitro </i>as a bedside device, within catheters for continuous, real-time measurements, or as an <i>in vivo </i>implant. Research has advanced via the glucose model to minimize costs and to explore how LbL designs need to be adapted when moving to an electrode with a wire geometry. Results of the model glucose LbL system on wire electrodes are presented, which will influence how the strategy will be used for other systems, such as uric acid sensing, which would immensely benefit in clinical applications if successfully implemented at wire electrodes.

Development of layer-by-layer design of xerogel-based amperometric first generation biosensors on wire electrodes

253rd National Meeting (2017)

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