Eukaryotic cellular signaling networks are composed of highly interconnected systems of regulatory proteins, which control nearly all biological processes. Protein post-translational modification plays a central role in these networks, and of particular interest is phosphorylation mediated by protein kinases and phosphatases. With this in mind, we are studying these signaling pathways using both phosphoproteomic and transcriptomic approaches. As a model, we are using the filamentous fungus <i>Aspergillus nidulans</i>, studying the cell wall integrity signaling (CWIS) pathway. The CWIS pathway consists of a MAPK cascade (BckA-MkkA-MpkA), regulates alpha-glucan synthase genes, and is understood to be induced on cell wall perturbation. When comparing wild type fungi to a MAPK deletion mutant (<i>ΔmpkA</i>; CWIS deficient), our combined protein and transcript data imply this pathway is involved in a number of other, previously unknown, cellular pathways. We will discuss these alternate pathways and how CWIS pathway components may be involved.

Phospho-proteomic and transcriptomic data for fungal cell-wall repair pathway reveal new functionality

253rd National Meeting (2017)

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