Literature regarding protein amyloid fibril inhibition has demonstrated the capability of two polyphenolic drugs, Curcumin and Epigallocatechin-gallate (EGCG), which limit or fully inhibit amyloid aggregation in a variety of fibrillation models including the hen egg white lysozyme model (HEWL). However, they need to be protected from the physiological barriers of the GI tract to enhance drug absorption and bioavailability. Methods regarding the encapsulation of these compounds are well established in a variety of drug delivery systems. The inhibition, disaggregation and destabilization effects on the HEWL fibrillation model have been studied in isolation for these compounds, and therefore the combined effect merits further characterization, considering their synergistic roles in a wide array of pathologies. Considering this we are interested in developing multilayer alginate bead’s loaded with these two natural polyphenolic compounds in such a manner that their release can be controlled and their integrity maintained while travelling down the GI tract ensuring greater release kinetics in intestinal fluids and enhancing bio absorption. To accomplish the proposed objectives, the synthesis of immobilized Curcumin-ECGC multilayer sodium alginate beads (Cur-ECGC–M–ALG–Beads) will be prepared by external ionic gelation and Layer-by-Layer (LBL) modified method. Following with the characterization using optical microscopy, electron microscopy (SEM), and FTIR. To measure loading efficiency and release of drugs, we will use UV–vis spectrophotometry and FTIR. Examination of the dual inhibition effect of Curcumin and Epigallocatechin-gallate on amyloid fibril formation after the release from the loaded beads, in a HEWL fibrillation model wil be studied using spectrofluorometric technique, Thioflavin T (ThT) fluorescence and Dynamic Light scattering (DLS) to follow the change in the size of aggregates.

Disaggregation of lysozyme amyloid fibrils by combined drug delivery of curcumin and epigallocatechin gallate via multilayer Ca-alginate hydrogel micro-encapsulation

253rd National Meeting (2017)

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