Formation of liposomes of complex lipid compositions in high ionic strength buffers is challenging with the techniques that are used currently. This poster reports on our studies of the effects of varying ionic strengths of the buffer solution and the lipid composition in the newly developed PAPYRUS technique (Paper-Abetted liPid hYdRation in aqUeous Solutions). We find that a two-step procedure, where a concentrated ionic stock solution is added after an initial growth period resulted in greatly improved yield and size of liposomes through PAPYRUS. We discuss our results based on the importance of electrostatic repulsion on the mechanism of growth of liposomes on paper-based substrates, and demonstrate the incorporation of membrane-associated proteins in the liposomal membranes and soluble proteins within the lumen of the liposomes.

Paper-based growth of liposomes and proteoliposomes using PAPYRUS in ionic buffers

253rd National Meeting (2017)

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