The discovery of green fluorescent protein (GFP) has fundamentally transformed biological spectroscopy and imaging, allowing a wide range of biochemical and biophysical processes and interactions to be studied at the cellular and molecular level. However, the stringent conditions required for the fluorophore of GFP (and its derivatives) to mature can limit its applications. In this talk, we will describe a blue florescent amino acid (BFAA) that does not require a specific environment and additional time to form, has a large fluorescence quantum yield, long fluorescence lifetime, and good photostability. Specific examples will be discussed, showing the potential utility of this BFAA for investigating the structure and dynamics of membrane proteins via single-molecule spectroscopy or microscopy.<br/>

Blue fluorescent amino acid for studying membrane protein structure and dynamics

253rd National Meeting (2017)

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