Burkholderia fungorum is a plant pathogenic gram-negative bacterial species within the Burkholderia family. Members of this family are known for their antibiotic resistance, but very little is known about B. fungorum. To look for changes in outer-membrane (OM) permeability and thus glean information about antibiotic resistance a transposon will be utilized to create insertion mutants. They will then be screened against antibiotics that could potentially indicate changes to the OM. Utilizing the known transposon will identify the location of the insertion and characterize the cause of the antibiotic sensitivity.

Investigating B. fungorum antibiotic resistance with transposon mutagenesis

253rd National Meeting (2017)

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