TEV is used as a biological pair of scissors for the trimming of fusion proteins. TEV uses a highly selective active site which targets a specific sequence for action. TEV is widely used yet its activity is not well understood. Site directed mutagenesis was carried out to create a TEV mutant with equal efficiency but a different and distinct recognition sequence. The TEV mutant further explains TEV’s activity and can be used as an alternate trimming method.

TEV mutant production

253rd National Meeting (2017)

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