Various fungal species occupy environments both indoor and outdoor. There are even toxic species as well as harmless species to health. Thus, it is important to distinguish these fungal species. Although DNA sequencing-based methods are well established for identifying species, there are many other efforts with global bio-identification system so-called DNA barcode. According to other groups reports, among the six DNA regions evaluated as potential DNA barcodes for fungi, internal transcribed spacer (ITS) region has the highest probability of successful identification for the broadest range of fungi. ITS region is universal for fungi and distinguishing enough to identification species. In this study, we have investigated ITS region of fungal DNA as DNA barcode. Furthermore, we analyzed ITS1 and ITS2 divided by 5.8S rDNA, and we found that ITS1 region is a more effective distinguishing region for DNA barcode. Because conventional agarose electrophoresis method with DNA molecular weight marker has a poor resolution for identifying ITS region, we also analyzed ITS region with microchip electrophoresis (ME). With ITS region by ME analysis, we can get more distinguished ITS region gaps and categorize fungi at the genus level.

Identification of fungal genus level using DNA barcode method

253rd National Meeting (2017)

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