Natural Organic Matter (NOM) has shown to influence nanoparticle interactions with organisms. Studies with model supported lipid bilayer with lipopolysaccharides has demonstrated that at higher NOM concentrations, the interactions of lipid bilayers with poly(allylamine hydrochloride) functionalized nanodiamond (PAH-ND) decreased. The purpose of this work is to examine the modified interactions by NOM at a whole cell level, using a model gram-negative bacterium, Shewanella oneidensis MR-1. To test this hypothesis, bacterial viability was first assessed by colony counting and growth-based viability assays. BacLight Live-Dead assay was also utilized to investigate bacterial membrane damage. Together with ND Zeta potential results at varying NOM concentrations, we observed that a decrease in both toxicity and membrane damage by PAH-ND to S. oneidensis occurred at a similar NOM level where a charge reversal of the ND was observed.

Impact of natural organic matter on the interaction of functionalized diamond nanoparticles with model bacterium S. oneidensis MR-1

253rd National Meeting (2017)

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