There is a pressing need to discover new antibiotics to combat serious and life threatening infections. An attractive target is the methylerythritol 4-phosphate (MEP) pathway, which is used by most bacteria to produce isoprenoids.  The enzyme IspF is the fifth enzyme of the MEP pathway and contains a zinc ion.  A series of 2-amino-pyrimidine derivatives were designed to potentially bind to the zinc ion present in the IspF enzyme.   Differential scanning fluorimetry, surface plasmon resonance, and saturation transfer difference NMR techniques were used to evaluate binding.  Antimicrobial activity was demonstrated by Kirby-Bauer disk diffusion assays against nine strains of bacteria.

Evaluation of 2-amino-pyrimidine derivatives as antimicrobial agents and IspF inhibitors

253rd National Meeting (2017)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.