The effective monitoring or determination of multiplex pathogenic bacteria simultaneouls is very necessary and important for the solving some serious public issues (such as food safety control) caused by various pathogenic bacteria. In comparison to the conventional batchwise analysis model, a strategy that achieves simultaneous detection of multiple targets from complex samples could offer several advantages, including high-throughput, short assay time, and low cost. In this study, by employing quantum dots(QDs) and Fe3O4 nanoparticles as optical and magnetic components respectively, a series of fluorescent-magnetic multifunctional nanospheres with different magnetic susceptibility were constructed based on of self-assembly by metal coordination interaction. As pathogenic bacteria can cause serious infectious diseases, which threatens the health or even life of human beings. Then aptamers corresponding to specific pathogenic bacteria were coupled to the above-mentioned FMMNs to generate magnetic-encoded fluorescent multifunctional nanoprobes (MFMNprobes). Based on the MFMNprobes, three different kinds of pathogenic bacteria, S. typhimurium, Vibrio parahaemolyticus, Staphylococcus aureus, could be separated one by one from complex sample and then subjecting fluorescent detection in few minutes. The MFMNprobes based analysis strategy for multiple bacteria is simple, accurate, sensitive, and rapid, which hold the great potential in some pathogenic bacteria related important field like food safety control, clinical diagnosis and so on.

Magnetic-Encoded fluorescent multifunctional nanoprobes for multiplex pathogenic bacteria assay

253rd National Meeting (2017)

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