Surface topographic modifications, especially the control of surface roughness, have driven improved success rate of dental and orthopedic implants. In bone tissue engineering, silica nanoparticles (SiNPs) have been superimposed to modify surface properties with their potential ability for enhancing new bone formation. Herein we describe a protein-based roughness-tunable SiNPs nanostructure assembly for osteopromotive surface functionalization of titanium implants. A recombinant mussel adhesive protein fused with silica precipitating R5 peptide (R5-MAP) enabled efficient coating with strong adhesion ability, allowing de novo SiNPs formation under mild conditions. We exploited the multilayer assembly of R5-MAP-based SiNPs nanostructure (R5-MAP@SiNPs) onto titanium surface for the direct control of micro-scale roughness by adjusting the number of assembled layers. This multilayer assembly significantly increased in vitro osteogenic cellular behaviors of pre-osteoblasts on titanium surface in a roughness-dependent manner. The implantation of R5-MAP@SiNPs multilayer-assembled titanium surface relevantly induced not only a large amount of new bone formation, but also a high degree of bone maturation within a calvarial defect site, indicating an in vivo osteopromotive potential. Collectively, this modular nanostructure assembly can be practically applicable to accelerate bone tissue growth for improved stability and prolonged lifetime of medical implantable devices.

Silica nanostructured multilayer assembly using engineered mussel glue for accelerating bone growth on titanium implants

253rd National Meeting (2017)

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