Generation of stable high-yielding cell lines with confirmed monoclonality while keeping an accelerated timeline continues to be a challenge, especially with the increased demand for novel investigational therapeutics in the industry. While conventional cell line development (CLD) methods have been very successful in meeting most of the important industry demands, generation of acceptable cell lines using high-throughput automated platforms has been challenging. This case study evaluates the development of a high-yielding stable and monoclonal cell line generation platform based on single round of cloning using the ClonePix. Unlike previous attempts, this study compared the generation of cell lines with the above desired characteristics for CHO cells including CHO-DG44 and CHOK1 host cells. In addition the study also compares the ClonePix based single round cloning platform to that of the conventional high-throughput methods such as Fluorescence Activated Cell Sorting (FACS) CLD protocols. For this study both monoclonal antibodies and Fc fusion proteins were evaluated for generation of cell lines.<br/>Overall, the integrated approach to cell line generation at KBI will enable setting industry standards for generation of methods and protocols using ClonePix for single round cloning based cell line development.

Accelerated generation of high-yielding monoclonal stable CHO cell lines using a ClonePix-based cell line development platform

253rd National Meeting (2017)

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