Catechins, a flavanol and antioxidant, have been thought to be beneficial to overall human health for a small time now. There is reason to believe they are capable of inhibiting tyrosinase, the enzyme that catalyzes the first major step in the synthesis of melanin converting L-tyrosine into dopaquinone. The current project isolated catechins and concentrated them from Granny Smith Apples via amalgamation, filtering, and distillation. The presence of catechins in the apples was confirmed via UV-Vis spectroscopy. Utilizing a catechin standard, a curve was constructed so that the concentration of catechin per gram of apple could be calculated. The effect of these isolated catechins on tyrosinase was monitored in an assay containing a standard volume of tyrosinase and L-tyrosine via UV-Vis Spectroscopy. The type of inhibition observed by catechin was also determined.<br/>

Analysis of tyrosinase inhibition with extracted catechins from granny smith apples

253rd National Meeting (2017)

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