Micro-patterning cell scaffolds permit fruitful applications such as controlling of cellular shapes, precise monitoring molecular events in biochemistry, and drug screening. Here, by using inkjet printing of cytocompatible chitinous nanocrystal (NC) inks, we demonstrate microscopic localization of cellular adhesion. The bio-NC materials including cellulosic one have currently attracted attention and their dimensions are actually comparable with those of pigments for practical inkjet printers. We display how to regulate the occupied area and maximum height of the chitinous NC microstructures (dots and lines) using a research inkjet printer. Cellular adhesive capability was much improved for deacetylated chitin NC (dChNC), as already known for chitinous bulk materials. The dChNC remained nanocrystalline shape even after severe deacetylation and exhibited wide-angle X-ray diffraction pattern of chitosan. The obtained cellular adhesive dChNC ink may be digested enzymatically but non-proteolytically, therefore, essentially gives rise to no damage on cells when removing from the substrate. This is a promising approach for tailor-made tissue engineering.

Non-proteolytically digestible micro-patterning cell scaffolds by inkjet-printing of chitinous nanocrystals

253rd National Meeting (2017)

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