In light of the ever increasing demand for higher production capacity, there is a quest for increasing overall throughput to produce therapeutical proteins through process chromatography. Of the factors determining throughput, media capacity and selectivity are closely examined, as they play a major role in the purification process. We have showed earlier that multimode chemistry is advantageous for separating closely related proteins. The type of matrix and its properties such as backbone chemistry, pore structure, and linker can have significant impact on selectivity and capacity for the same type of ligand. In this study, we will show the effect of matrix on the separation of proteins using multimode ligands comprising strong cation and anion functionality on agarose, silica and polymethacrylate matrix with polyethyenamine and polyallylamine linkers.

Effect of matrix on protein separation and capacity with multimode ligands

253rd National Meeting (2017)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.