Developing a non-invasive, selective amperometric sarcosine sensor with high sensitivity has significant implications for prostate cancer detection and diagnosis. First generation biosensing schemes and direct electrochemical detection of sarcosine are both explored. As part of this work, the production and purification of sarcosine oxidase (SOx) in <i>Escherichia coli</i> was analyzed and optimized in order to maximize SOx functionality and affordability for 1<sup>st</sup> generation schemes with layer-by-layer construction. In these cases, sarcosine levels can be detected via amperometry measuring the hydrogen peroxide by-product of the enzymatic reaction at an electrode modified with immobilized Sox and supplemented by specific nanomaterials. Similarly, sensor designs for direct electrochemical detection of sarcosine at electrodes modified with carbon nanotubes are also explored.

Design and development of sarcosine sensors utilizing nanomaterials

253rd National Meeting (2017)

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