D-Serine acts as an endogenous co-agonist for N-methyl-D-aspartate receptors at synapses, making it essential for proper brain development and function. It has also been linked to several neurodegenerative diseases such as Alzheimer’s disease and dementia. Nevertheless, the primary site and mechanism of D-serine release remains unclear. We recently demonstrated the use of an enzymatic amperometric biosensor for the <i>in vivo</i> quantification of endogenous D-serine release in <i>Xenopus laevis</i> tadpoles. Herein, we investigate the effect of the permselective polymer layer thickness on the biosensor’s response time and selectivity. Scanning electrochemical microscopy (SECM) is then used with the optimized biosensor in order to measure localized release of D-serine from a model system. This SECM methodology, which provides high spatial and temporal resolution, could be useful to investigate the primary site and mechanism of D-serine release in other biological samples.

Localized detection of D-serine using scanning electrochemical microscopy and an enzymatic biosensor

253rd National Meeting (2017)

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