The fungus Macrophmina phaseolina leads to the charcoal rot disease on many plant species. Especially, it causes economic loss in agricultural products such as corn, cotton, soybean and sweet potato. Early detection of the present of M. phaseoline is important in disease prevention. One emerging detection method involves in developing chemical sensors that can monitor the microbial volatile organic compounds (MVOCs) as biomarkers released from different fungi isolates. Our preliminary data from the previous study showed that the variations of MVOC profiles among M. phaseolina from different hosts are significant. These variations may be caused by biological factors such as genetic mutation induced by microbiological differences between hosts. Therefore, further MVOC analysis of M. phaseolina isolates from identical hosts is required. In this work, we present the fundamental study in the detection of MVOCs emitted from M. phaseoline isolates collected from sweet potato. The preliminary results indicate that the MVOCs pattern from morphotype flat and fluffy isolates are different. The experimental parameters such as pH, a carbon source that affect the fungi growth were also analyzed. This study provided the fundamental information of M. phaseoline MVOCs emission as the first step in the chemical sensor development.

Detection of microbial volatile organic compounds released from Macrophmina phaseolina by headspace solid phase mirco-extraction and gas chromatography-mass spectrometry

253rd National Meeting (2017)

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