Upconverting nanoparticles (UCNPs) composed of NaYF4 doped with 30% Yb3+, 0.5% Tm3+ were covalently immobilized on aldehyde modified cellulose paper via reductive amination to evaluate the multiplexing capacity of luminescence resonance energy transfer (LRET) between UCNPs and quantum dots (QDs). This first account of a multiplexed bioassay strategy for the simultaneous detection of three analytes demonstrates the principle of use of a single form of UCNP as donor and three different color emitting QDs as acceptors. Broad absorbance profiles of green, orange and red QDs overlap with a blue emission band from UCNPs allowing for LRET. The narrow and well-defined emission peaks of UCNPs and QDs allowed for the collection of luminescence from each nanoparticle using band-pass optical filters and an epi-fluorescence microscope with limits of detection of 10 fmol. UCNP luminescence lifetimes are also studied.

Multiplexed resonance energy transfer nucleic acid hybridization assay using a single form of upconversion nanoparticle as donor and three quantum dots as acceptors

253rd National Meeting (2017)

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