Curcuminoids are a class of photoactive organic compounds that have been found to have practical application in Photodynamic Drug Therapy (PDT).  To understand the practicality of a given curcuminoid in PDT the binding relationship to the protein Human Serum Albumin (HSA) at physiological pH 7.4 must be identified.  This particular parameter can be analyzed through a variety of photophysical studies.  In particular, quenching studies and the use of a Stern-Volmer plot provides insight to the K<sub>sv</sub> value and in turn, the determination of the K<sub>b</sub> (binding constant).  Another parameter of interest is the number of binding sites between a given curcuminoid and HSA.  This can be analyzed through the logarithmic relationship between fluorescence intensities and curcuminoid concentrations.  Alternative and future methods of analysis include Benesi-Hildebrand plots and identification of thermodynamic parameters.  The data obtained thus far indicates that there is consistently one binding site for the curcuminoids within HSA.  Further analysis will provide more concrete information pertaining to the overall binding coefficients and stoichiometric relationships.

Analyzing the binding relationship between curcuminoids and HSA by steady state fluorescence spectroscopy

253rd National Meeting (2017)

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