Method validation is a process that is widely utilized in pharmaceutical industry to verify the reliability of an analytical method. A new experiment is designed to introduce the concept of method validation to senior undergraduate chemistry student, primarily for an analytical or instrumental laboratory course. Using HPLC system, a common over-the-counter painkiller, acetaminophen is analyzed. In general, method validation study usually includes specificity, linearity, accuracy, precision, range, detection limit, quantitation limit, and robustness. Due to the consideration of the length of the typical laboratory section, the design of this experiment is mainly focused on the linearity, accuracy, and repeatability of the method. The application of this experiment can help undergraduate students in the classroom to be exposed to method validation procedure.

Validation of acetaminophen using HPLC, an undergraduate experiment

253rd National Meeting (2017)

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