Fourier Transform Infrared (FT-IR) spectroscopy was used to acquire spectra (1800-650 cm<sup>-1</sup>) from a range of specially prepared calibration standards and test samples. A multivariate analysis approach was required to extract important information from the FT-IR spectra. Raw data were subjected to principal component analysis (PCA) to allow for the construction of appropriate scores plots and calibration curves. These were used in turn to predict the amount of bound mineral present in the test samples. A separate analysis of total mineral content by inductively coupled plasma optical emission spectroscopy (ICP-OES) allowed for the calculation of bound mineral as a % of total mineral content. The method was validated in accordance with regulatory guidelines and independently verified for the quantitative analysis of organically bound mineral in nutritional supplements.<br/>The non-destructive approach used overcomes some of the difficulties associated with assays that require dissolution or digestion of the test material before analysis. Certain challenges remain and the product-specific nature of the assay requires specially prepared calibrants for individual products within difference organic trace mineral classifications. Although a similar FT-IR/PCA approach may be suitable for other products and preparations, validation and verification studies are required to ensure that it is “fit for purpose” and suitable for application under specific conditions.

Quantitative analysis of organically bound minerals in nutritional supplements using FT-IR spectroscopy

253rd National Meeting (2017)

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