Ultra High Pressure Liquid Chromatography (UHPLC), with operating pressures in the range of 1,000 to 3,000 bars, permits operation of long capillary columns packed with particles between 1 and 2 micron diameter. In a collaborative research project between the Jorgenson group and the Tallarek group, we are determining some of the the underlying structural causes of poor column efficiency. We are using this knowledge to guide development of methods to pack increasingly efficient columns. This effort has recently resulted in production of capillary columns 100 cm long packed with 2 micron particles which produce 500,000 theoretical plates at the optimum velocity. This corresponds to a minimum plate height of 2 microns, or one particle diameter. The correlation of several characteristics of the packed bed structure with column performance will be discussed. Based upon this structural information, methods for the slurry packing of micron-sized particles to produce columns of exceptional separation power will be described.<br/>

Capillary LC with sub-2 micron particles: Effects of column packing conditions on column morphology and efficiency

253rd National Meeting (2017)

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