Nonlinear light scattering in the form of Second Harmonic Generation, due to its symmetry properties, has been proven effective for characterizing molecular transport through the membranes of living biological cells with membrane specificity and real time resolution. Second Harmonic Light Scattering (SHLS) has even been demonstrated as a label free method so that transport rates of molecules, with or without nonlinear polarization, can be determined. Our studies based on SHLS have shown dramatic differences in transport rate due to differences in molecular structure and membrane modification. In this report, we show our effort in developing SHLS as a microscopic tool for imaging molecular transport through living biological cells with time and spatial resolution.<br/>

Time-resolved second harmonic microscopy of molecular transport through living cell membranes

253rd National Meeting (2017)

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