<br/>There is growing interest within the biopharmaceutical industry to develop processes which improve individual unit operation performance and overall process train efficiency through linked and continuous processing. The use of single pass tangential flow filtration (SPTFF) technology can help facilitate these process intensification efforts by modifying process intermediate volumes and concentrations without the need for recirculation. In this work, SPTFF is used prior to a flowthrough anion exchange (AEX) mAb polishing step to boost resin loading and productivity. Results are presented which show that linking SPTFF with AEX chromatography provides improvements in both host cell protein impurity clearance and process economics as compared to using AEX alone. Excellent removal of MVM and XMuLV is also maintained at the optimized conditions. Considerations for developing and implementing this approach as either a batch step or combined with others as part of a continuous process are also discussed.<br/>

Linking single pass tangential flow filtration with anion exchange chromatography for intensified mAb processing

253rd National Meeting (2017)

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