Hydrogen sulfide (H2S) is well-known for its unpleasant smell of rotten eggs and has been considered as an environmental toxic gas for centuries. However, recent investigations have demonstrated that H2S is the third endogenously produced gaseous signaling molecule, plays an increasingly crucial role in a variety of physiological processes. It has been demonstrated that the abnormal H2S level in cells is correlated with the symptoms of Down syndrome, Alzheimer’s disease, Parkinson’s disease and febrile seizures. Accordingly, sensitive and selective detection techniques that enable the content of H2S in complicated biological systems to be probed are highly valuable. Herein, we report a new, 3D microfluidic paper-based turn-on electrochemiluminescence origami cyto-device with hollow channels for analysis of H2S released from living cells (Scheme 1). Using 3D macroporous Au@Pt core-shell nanoparticles-paper electrodes as the working electrodes and efficient platforms, aptamers modified blue-luminescent graphene quantum dots (bGQDs) were modified for the specific cancer cells capture. The bGQDs showed high ECL efficiency with K2S2O8 as a coreactant and quenched after reaction with Cu2+. The cyto-sensor demonstrated enhanced ECL signal after reacting with H2S or S2− ions due to the high-affinity binding between sulfur and Cu2+, which leads to sensitive recording of the H2S level in cellular environments. Inspired by mentioned above, a simple and effective sensing platform for detection of cellular H2S released from MCF-7 cells was demonstrated based on signal-on strategy of H2S to Cu2+-GQDs, which has potential utility to help understand the regulation of H2S in physiological and pathological events.

Lab-on-paper cyto-device for the detection of H2S released from MCF-7 cells

253rd National Meeting (2017)

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