The ability to monitor and manipulate the microenvironment of cells and tissues is one of the most promising applications for microengineered systems. The laboratory is developing a suite of technologies based on microengineered platforms and microfluidics to manipulate and analyze living cells and organoids. We have developed simple, inexpensive fabrication methods utilizing photoresists, plastics, and hydrogels to array cells and organoids. The fabricated devices include detachable, deformable, or biodegradable array elements designed for cell/organoid analysis and sorting. Cells cultured in these engineered microenvironments yield high-density arrays for high through put analyses and assays followed by isolation of individual cells and organoids.  The arrays bring the power of cell sorting capabilities to high-content-screening microscopy.  Applications for this technology are envisioned for the sorting of single cells for mRNA expression analysis, identification and capture of rare immune cells for immunotherapy, colony biopsy and screening of primary samples using complex phenotypes as separation criteria.

Microfabricated systems to screen and separate cells and tissues based on complex phenotypes

253rd National Meeting (2017)

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