Blood orange juice is one of the most popular citrus juices, owing to both its rich flavor and its unquestionable health promoting properties derived from a wide variety of components, ranging from vitamin C to flavonoids (e.g., flavanones, flavones, flavonols and anthocyanins).<br/>As a part of our ongoing studies on the composition and properties of juices obtained from citrus species grown in the Mediterranean basin, we have reported recently on the flavonoid content of the juices from Moro, Sanguinello and Tarocco pigmented sweet orange varieties, thus identifying and quantifying a range of <i>C</i>-glucosyl flavones (such as lucenin-2, vicenin-2, stellarin-2, lucenin-2 4'-methyl ether and scoparin) and a 3-hydroxy-3-methylglutaryl flavonol derivative (namely, 3-hydroxy-3-methylglutaryl-glycosyl quercetin) by means of HPLC-DAD-MS-MS analysis.<br/>Given the limited data available in the literature on the influence of these 'minor' components on the biological effects, we have started to evaluate their potential health-promoting abilities by assaying their antioxidant (FRAP assay and DPPH, superoxide anion, hydroxyl and ABTS radical scavenging assay) and cytoprotective activity, as well as their potential to inhibit microorganism growth. Preparative RP-HPLC separation allowed us to gain further information on the contribution of these flavonoids to the total activity of crude blood orange juices.

C- and O-glycosyl flavones in pigmented orange juices: Identification, quantification and evaluation of their influence on antioxidant and cytoprotective activity

253rd National Meeting (2017)

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