Next-generation plant-incorporated-protectants in genetically modified crops rely on RNA interference (RNAi): these crops express double-stranded RNA (dsRNA) biopesticides that, upon ingestion by a pest insect feeding on the crop, interfere with the biosynthesis of specific and essential proteins in the insect, typically resulting in mortality. Some dsRNA biopesticides are rapidly approaching commercialization. However, limited information is available on the processes that govern the fate of dsRNA biopesticides in environmental matrices, resulting in uncertainty in exposure analysis required for environmental risk assessment. To reduce these uncertainties, we have developed approaches to evaluate the adsorption and transformation of dsRNA biopesticides and non-insecticidal model dsRNA in environmental matrices including soils, sediments and surface water bodies. Experimental results collected using model dsRNA investigated the contribution of microbial and enzymatic degradation, abiotic transformation, and adsorption to particle surfaces to dsRNA fate in these environmental matrices. The presentation will highlight the application of new experimental approaches to overcome methodological and conceptual challenges associated with studying the fate of biomacromolecular pesticides.

Environmental fate of double-stranded RNA (dsRNA) biopesticides from RNA interference (RNAi)-based crop protection

253rd National Meeting (2017)

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