The determination of an efficient preparative gradient method for C18 from analytical data is not simply scaled up from an analytical scouting run. This is due to the fact that the actual solvent composition eluting the compound during the purification run is delayed from the value observed in the HPLC/UPLC scouting gradient. An algorithm that calculates the focused gradient parameters for a preparative column using the data obtained from an HPLC/UPLC scouting gradient is described. The calculated focused gradient saves time and solvent when compared to the preparative system default gradient conditions. It also eliminates the guesswork in how to produce a fast, efficient gradient manually. The algorithm is also applicable to other adsorption chromatography techniques such as flash chromatography (both normal and reverse phase) as well as supercritical fluid chromatography (SFC).<br/>

Preparative method development from analytical columns

253rd National Meeting (2017)

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