Surface Plasmon Resonance (SPR) is a popular method to measure molecular interactions, such as drug and protein affinity and binding kinetics in real-time and label-free. Multi-Parametric Surface Plasmon Resonance (MP-SPR) extends applicability of SPR from molecular interaction measurements to more fundamental studies of nanoparticle interactions, and materials characterization. The MP-SPR instruments can perform measurements in a wide angular range and can record SPR curves at more than one wavelength, characterizing nanoscale films for both thickness and refractive index. In situ deposited polyelectrolyte multilayer, consisting of polystyrenesulfonate and polyallylaminehydrochloride (PSS:PAH), and CVD grown graphene layer were characterized using MP-SPR. Conformation of deposited lipid bilayers and vesicle layers were confirmed using thickness and refractive index information. 100% serum sample interaction and corona formation on liposome surface was studied using MP-SPR. The mass of adsorbed molecules on the polymer surface and the best antifouling surface was determined. Recently the mean size of bound nanoparticles was determined using the technology. The MP-SPR method has shown good correlation with reference methods used in the studies such as quartz crystal microbalance (QCM), atomic force microscopy (AFM) and Raman, and with previous literature values for similar systems. High sensitivity of the method enables characterization of layers down to subnanometer thickness. As an non-invasive method without need for measurements in vacuum, MP-SPR is proven to be a fast and effective tool for characterization of nanoparticle interactions and nanoscale thin films in air and in liquid.

Characterization of nanoparticle interactions and thin film properties using MP-SPR

253rd National Meeting (2017)

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