Salmonellae are among the leading causes of foodborne outbreaks in the United States, and more rapid and efficient detection methods are needed. Surface plasmon resonance imaging (SPRi) is an emerging optical technique, which allows for rapid and label-free screening of multiple targets simultaneously. In this study, we have evaluated the feasibility of SPRi in label-free detection of Salmonella isolates. The SPRi-biochip was functionalized with anti-Salmonella antibodies and blocking agents. Intact and thermally lysed Salmonella, E. coli, Listeria innocua, and Staphylococcus aureus cells were tested for performance of SPRi as a new tool for foodborne pathogenic bacteria detection. The influence of antibody concentration, blocking agent, and regeneration conditions on specificity and signal intensity for target bacteria were evaluated and optimized. Salmonella could be detected based on SPR sensorgram analysis and difference images, and signal from lysed cells yielded significantly higher signal compared to intact cells. Overall, the new SPRi technique demonstrates the potentials in bacterial food safety and could lead to significant impacts on future pathogen screening in food matrix.

Multiplex surface plasmon resonance imaging platform for label-free detection of foodborne pathogens

253rd National Meeting (2017)

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