This project illustrates the effects of nine osmolytes on the partitioning thermodynamics of caffeine between aqueous and cyclohexane phases. The stabilizers such as betaine and sarcosine decrease the Gibbs free energy for caffeine transfer and enhance caffeine transfer from the aqueous to cyclohexane phase. On the other hand, the denaturants such as urea and guanidinium salts behave the opposite to hinder the caffeine transfer. Gibbs free energy for caffeine transfer was measured at different temperatures to obtain the enthalpy and entropy for caffeine transfer. Caffeine transfer from the aqueous to cyclohexane is entropically driven, though the differences between osmolytes arise primarily from the enthalpy of caffeine transfer. <sup>13</sup>C and <sup>1</sup>H NMR spectroscopy were employed to determine the specific interactions of each osmolyte with caffeine molecule.

Effects of osmolytes on caffeine partitioning thermodynamics

253rd National Meeting (2017)

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