The human SULF1 gene codes for the enzyme endo sulfatase 1, SULF1, which is located in the extracellular matrix of cells. Current research indicates that the concentration of SULF1 in body fluid is highly influenced by the carcinogenous process, especially in the early stages of breast cancer. Compared to normal, healthy controls, SULF1 gene expression has been discovered to increase to levels up to 40 fold in breast cancer patients. Thus, developing a method to efficiently and effectively monitor the SULF1 level would greatly aid in the pursuit of finding a successful way to detect breast cancer in its early stages. In this project, an electrochemical approach is able to be used to determine the concentration of electrochemical probe contained sulfate groups before and after SULF1 is added in order to determine its properties such as its reaction rate, optimum pH, and temperature of the enzyme. As a result, utilizing the properties of the SULF1 enzyme can lead to a rapid and noninvasive method for point-of-care breast cancer diagnosis.

Determining extracellular sulfatase SULF1 concentration as a biomarker to detect the onset of breast cancer using an electrochemical approach

253rd National Meeting (2017)

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