Microfluidics and the concept of lab-on-a-chip continue to gain traction as a successful emerging field that aims to integrate complex biochemical analyses into automated systems. One of the most promising applications for these microfluidic systems is in point of care blood diagnostics. Isolation of cells, such as circulating tumor cells from blood provides insights for diseases diagnosis, developing drugs and treatment. Several approaches have been used to capture cells using microfluidics devices. However, many of these approaches don’t yield viable cells and change the phenotype of cells by activating the cells, which negatively affects the downstream processing. Layer-by-layer (LbL) assembly offers an alternative method for surface modification, for the fabrication of uniform and stable coating of microfluidics devices. LbL is a highly versatile and simple procedure that has been employed in microfluidics in many applications and also for in-vitro assay for studying fundamental tissue and cell biology. Naturally occurring polymers are widely used in LbL coating. In this work, we introduce for the first time LbL coating of cellulose nano fibrils (CNF) in the microfluidic channel for efficient cell capture. We report here the efficient capture of viable cells. In addition, we demonstrate efficient release of the captured cells from the microfluidic devices. This LbL coating strategy is stable and may be used for capturing of different cell types from complex biological fluidics, such as whole blood. Finally, we demonstrate proliferation of released cancer cells and cell viability, which opens up for applications in cancer biology

Nanocellulose mediated layer-by-layer chip modification for cellular in-vitro diagnostics

253rd National Meeting (2017)

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