RNA-RNA interactions are essential in regulating gene expression. To identify RNA-RNA interactions transcriptome-wide, we aim to use psoralen to crosslink pyrimidines within and between RNAs in live cells, and isolate the crosslinked RNAs. To distinguish between bona fide trans interactions from long-range cis, the RNAs will be labeled with two orthogonal nucleotide handles. Through the use of biochemical purification techniques based on these handles, we isolate the crosslinked trans RNA interactions. This novel approach will map RNA-RNA interactions on a transcriptome-wide scale and lead to a better understanding of the underlying mechanisms of RNA networking in biology.

Transcriptiome-wide mapping of RNA-RNA interactions

253rd National Meeting (2017)

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