C-reactive protein (CRP) is an acute phase protein which has been widely used as a predictor of cardiovascular disease. We report herein the generation of cellulose nanofibrils (CNF) based immunosensors for CRP detection using quartz crystal microgravimetry (QCM). Ultrathin film of carboxylated CNF on the QCM chip was prepared via two methods: (i) CNF was firstly spin coated to QCM sensor, and then carboxylated using 2,2,6,6-tetramethyl-piperidine 1-oxyl (TEMPO)-oxidation method to convert the hydroxyl groups on CNF to carboxyl groups or (ii) the pre-carboxymethylated CNF suspension was spin coated directly on QCM chip. A ligand, protein A was conjugated to carboxylated CNF via 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride/N-hydroxysuccinimide (EDS/NHS) coupling chemistry for the further oriented immobilization of anti-human C-reactive protein (anti-CRP). The two methods give different surface density of carboxyl groups, which further influence the binding amount of protein A and anti-CRP. The detection efficiency for CRP of surfaces with oriented and randomly immobilized anti-CRP was compared. The specificity of CNF immunosensor for CRP detection and detection range was also investigated.

Immunosensor based on cellulose nanofibrils for C-reactive protein detection

253rd National Meeting (2017)

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