Fenoxaprop-ethyl, cyhalofop-butyl and fluazifop-butyl are aryloxyphenoxypropionate herbicide classified widely used to control broadleaf weeds. The aim of this study was to development of analytical method for fenoxaprop-ethyl, cyhalofop-butyl, fluazifop-butyl and their metabolites (fenoxaprop, cyhalofop and fluazifop) in rice and pepper using LC-MS/MS with negative and postive electrospray inonization mode and selected reaction monitoring. Kinetex C18 analytical column (2.1 x 100 mm, 2.6 <i>µ</i>m) are used for peak separation. The mobile phases were methanol and water containing 0.1% formic acid and 5 mM ammonium formate. For the treatment of sample (10 g), 20 mL of acetonitrile containing 1% acetic acid was added to sample before shaking (20 min), and then 4 g of MgSO<sub>4</sub>/1 g of NaCl were added, shaken (1 min) and centrifuged (3,000 rpm, 10 min). The method limit of quantitation (MLOQ) was 10 ng/mL under matrix matched calibration. A recovery test was performed at two fortification levels (10 MLOQ and 50 MLOQ) to validate the established method. The recovery rate was in the range of 70~120% with 10 % of coefficient of variation, proving the developed method is suitable for quantitative determination of those pesticide and metabolites.

Development of the simultaneous analytical method for aryloxyphenoxypropionate herbicides and their metabolites in rice and pepper using LC-MS/MS

253rd National Meeting (2017)

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