Polyhydroxyalkanoate (PHA) is a natural polyester synthesized by Pseudomonas putida as intracellular granules for energy storage. Soybean-based vegetable oil or dextrose was used as the carbon source, and cultures were prepared in shake flasks for 5 days before centrifugation and extraction with hot chloroform for 3 hours. The majority of the chloroform was removed by distillation, and then PHA was precipitated out by mixing with methanol. The PHA obtained had a melting point of about 166 oC, and the cell dry weight concentration was less than 1 g/L for soybean-based vegetable oil and about 3 g/L for dextrose as the carbon source. The NMR spectra, melting point measurements, microbial growth curves, and percent yield of PHA will be presented.

Biosynthesis of polyhydroxyalkanoate with Pseudomonas putida

253rd National Meeting (2017)

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