Chlorine dioxide has been use as an effective disinfecting agent in hospitals, food manufacturing facilities and water treatment plants. This study investigates the biocidal effects of gaseous chlorine dioxide on Escherichia coli K-12 cells in media solutions. The main focus of this research is to determine the concentration of chlorine dioxide and the exposure time needed to kill up to 2 x 109 cells.  E. coli cells were exposure to chlorine dioxide gas produced using 0.5 gram of precursor A and activator B for various time periods of  1.0, 1.5 and 2.0 hours.  The BacLight bacteria viability assay and florescence spectrometry technique was used to determine the ratio of live to dead cells in untreated and chlorine dioxide treated samples. A one-hour treatment at 0.88 mM chlorine dioxide inhibited growth of about 59.41 % of the treated E. coli cells whereas exposure to high chlorine dioxide concentration of 2.52 mM killed all 1.80 x 108 treated cells.<br/><br/>

Application of chlorine dioxide as a bactericide agent

253rd National Meeting (2017)

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