Amino acids play significant role in cell growth and productivity, and their optimization is critical in media formulation. Amino acid supplementation is usually optimized to meet process requirements. Mostly iterative and qualitative experimental approaches are used to optimize the amino acid concentrations. In this work, an unstructured kinetic model for optimization of amino acid concentration in cell culture media has been assessed. This model is built using gPROMS (PSE Enterprise, UK) and is based on 2 different types of kinetics- Monod type kinetics for cell growth and Leudeking-Piret type kinetics for protein production. The sensitivity analysis has been done on 64 model parameters and the critical parameters of the model have been evaluated by using experimental data from shake flask runs. This model would further be investigated and validated using different set of experimental data. This model would be potentially used to optimize the amino acid concentration in cell culture media with minimal experimentations for a new process. This will potentially reduce the time for media and feed optimization.

Kinetic modeling approach for media formulation optimization in mammalian CHO cell-cultures

253rd National Meeting (2017)

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