Protein dynamics at interfaces are crucial to a wide range of applications including disease inception, drug delivery, and separations science.  Recent efforts by our group and others have shown the promise of applying single molecule methods to link mechanistic detail about protein adsorptions to macroscale observables. However, although the best super-resolution methods achieve high spatial resolution, the temporal resolution remains low. We report Super Temporal-Resolved Microscopy (STReM) to improve the temporal resolution of 2D super-resolution microscopy by a factor of 20 compared to the traditional camera-limited frame rate. This is achieved by rotating a phase mask in the Fourier plane during data acquisition and then recovering the temporal information by fitting the PSF orientations. The feasibility of this technique is verified with both simulated and experimental 2D adsorption/desorption and 2D emitter transport.  When STReM is applied to measure protein adsorption at a glass surface, previously unseen dynamics are revealed.<br/>

Super Temporal-Resolved Microscopy (STReM) for measuring fast interfacial dynamics

253rd National Meeting (2017)

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