Chronic alcohol induces the expression of mitochondrial biogenesis genes as a compensatory mechanism against mitochondrial oxidative damage. The mechanisms by which chronic alcohol induces mitochondrial biogenesis genes are unclear. In this work, we evaluated the hypothesis that reactive oxygen species derived from CYP2E1 trigger the induction of mitochondrial biogenesis genes in hepatocytes. HepG2 cells overexpressing CYP2E1 (E47 cells, transfected with an expression vector containing the human CYP2E1 cDNA) and HepG2 cells not expressing any cytochrome P450 (C34 cells, transfected with an empty expression vector) were used in this study. Overexpression of CYP2E1 in E47 cells was confirmed by western blot, RT-PCR and enzymatic analysis. E47 cells showed higher expression at the mRNA level of the biogenesis genes TFAM and PGC1-alpha. These results show a correlation between CYP2E1 expression and expression of mitochondrial biogenesis genes. The mechanism by which CYP2E1 induces mitochondrial biogenesis is under further investigation in our lab.

Effect of CYP2E1 overexpression on mitochondrial biogenesis in HepG2 cells

253rd National Meeting (2017)

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