Microarrays based on protein chips are useful in a wide variety of applications that involve detecting and measuring protein-protein interactions, protein-small molecule interactions, and products generated by immobilized enzymes. Immobilized proteins are adsorbed on surfaces through non-covalent interactions or are tethered covalently. We have developed mild procedures for attaching proteins to silica and gold surfaces that are regio- and chemoselective and preserve the functions of the proteins. We will discuss the application of these procedures to antibody-binding proteins to give surfaces capable of immobilizing a variety of different antibodies and to construction of microarrays formed by microfluidic spotting.

Regio- and chemoselective immobilization of proteins on surfaces

253rd National Meeting (2017)

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