This work demonstrates that a lutecium orthosilicate (Lu<sub>2</sub>SiO<sub>5</sub>:Ce, LSO:Ce) radioluminescent nanoparticle can be coated with a dye and a dye coated polymer shell or a dye coated protein shell and still generate an effective energy transfer between the dye and core when excited via light or X-rays. LSO:Ce particles are functionalized with azides to facilitate click reactions between the particle and an alkyne modified fluorophores. This technology can be applied to attach an alkyne-functionalized dye to the particle, making this system highly tunable to the wavelength of interest. Specifically, alkyne functionalized napthalimide and rhodamine B have been used as the fluorophores of interest because they are a good FRET pair. Both dyes have been attached directly to the LSO:Ce particle and show effective energy transfer. Additionally, core/shell LSO:Ce/poly(propargyl methacrylate) nanoparticles have been synthesized and surface functionalized with napthalimide and/or rhodamine B to explore the possible energy transfer between the LSO:Ce core and the dyes. Indeed, there was an effective energy transfer between the core and dye for both particulate systems when excited via light and X-rays at the absorption of the LSO:Ce core. Furthermore, the energy transfer mechanism has been explored by using napthalimide dyes of various lengths. By understanding the energy transfer mechanism, the particles can be tuned to generate the maximum amount of light output. Applications of these radioluminescent particles include optogenetics, where neural firing is controlled via light through a microbial opsin, namely channelrhodopsin. Radioluminescent nanoparticles have the opportunity to replace damaging fiber optics currently used in optogenetic applications.

X-ray excited optical luminescence of surface functionalized, hybrid LSO:Ce-fluorophore particles

254th National Meeting (2017)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.